![]() †Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, United States. Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing.The overall protein architectures of these closely related proteins are implicated in contributing to the catalytic site differences. X-ray crystal structures of EcMTAN, VcMTAN, and a multiple-site mutant of VcMTAN most closely resembling EcMTAN in catalytic site contacts show no major protein conformational differences. The transition-state analogue affinity and thermodynamics in mutant VcMTAN became even more unlike those of EcMTAN, the opposite of expectations from catalytic site similarity thus, catalytic site contacts in VcMTAN are unable to recapitulate the properties of EcMTAN. Therefore, a single amino acid, Val153, alters the catalytic site cooperativity in VcMTAN. Knife Block With Divider
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